WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

Sabrin Ibraheem Mohsin, Salema Lafta Hassan* and Taghred Jabbar Humadai

ABSTRACT

The purpose of the current study was to examine the harmful effects of cygon in mice, as well as the impact ofimmunological stimulation with Aftovaxpur DOE vaccine and histopathological abnormalities. Eighty-four-weekoldadult Swiss Albino mice were split up into four groups. The first group received 4.5 mg/kg of Cygon orallyevery day for four weeks. The second group was given 0.1 ml of the Aftovaxpur DOE vaccine intraperitoneally(I/P) twice at two-week intervals while also receiving cygon orally every day for four weeks. I/P administered 0.1ml of the Aftovaxpur DOE vaccine to the third group. The negative control was assigned to the fourth group.Samples of blood were drawn from the first, second, third, and fourth groups in order to measure serum IL8, IL10levels using an ELISA kit. The results of ELISA tests for detecting IL8 and IL10 titers in group three(P≤0.05)demonstrated a substantial increase (P≤0.05) when compared to group two, group one, and group four.Clinicalsigns of treated mice showed depression and decreased appetite throughout the trial period, the anorexiaand weight loss were brought on by pathological lesions that resulted in intestinal malabsorption and stomachindigestion. Pathological lesions of the intestine showed desquamation in epithelial cells and mucus accumulationin addition to necrotizing enteritis also degeneration of mucous glands and inflammatory cells infiltration betweenmucous glands, as well as hyperplasia of goblet cells, in stomsch congestion of blood vessels and moderatehyperkeratosis in epithelial cellsEven so, the groups that received Cygon treatment and the Aftovaxpur DOEvaccination showed improvement against the negative effects of Cygon.