WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals
World Journal of Advance Healthcare Research (WJAHR) has indexed with various reputed international bodies like : Google Scholar , Index Copernicus , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , Cosmos Impact Factor , Scientific Indexing Services (SIS) , UDLedge Science Citation Index , International Impact Factor Services , International Society for Research Activity (ISRA) Journal Impact Factor (JIF) , IFSIJ Measure of Journal Quality , Scientific Journal Impact Factor (SJIF) , International Scientific Indexing, UAE (ISI) (Under Process) , International Impact Factor Services (IIFS) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

ISSN 2457-0400

Impact Factor  :  5.464

News & Updation

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in WJAHR coming Issue.

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • 6th International Conference on Human and Soci

    Venue:FCT Education Resource Center, Abuja- Nigeria                                        September 22-24, 2019

  • WJAHR New Impact Factor

    Its our Pleasure to Inform you that WJAHR Impact Factor has been increased from  4.897 to 5.464 due to high quality Publication at International Level

  • .

    6th INTERNATIONAL CONFERENCE ON EDUCATIONAL TECHNOLOGY AND CURRICULUM STUDIES(ICETC2019) 

     

    Venue: FCT Education Resource Center, Abuja-Nigeria

    September 22-24, 2019

  • WJAHR: NOVEMBER ISSUE PUBLISHED

    NOVEMBER 2021 Issue has been successfully launched on 1 November 2021

  • New Issue Published

    Its Our pleasure to inform you that, WJAHR 1 November 2021 Issue has been Published, Kindly check it on http://www.wjahr.com/home/current_issues

Best Paper Awards

World Journal of Advance Healthcare Research (WJAHR)Honored the authors with best paper award, monthly based on the innovation of research work. Best paper will be selected by our expert panel.

Best Article of current issue

Download Article : Click here

Indexing

Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METFORMIN HCl AND MYO-INOSITOL IN PHARMACEUTICAL DOSAGE FORM BY USING RP-HPLC

Dr. P. Nagaraju*, G. Parimala Devi

ABSTRACT

A simple, sensitive, specific, accurate reversed phase high performance liquid chromatographic method was developed for the simultaneous estimation of Metformin HCl and Myo-Inositol in pharmaceutical dosage form. RP-HPLC separation was achieved on an Enable C-18 250mm x 4.6mm, 5?m column. The mobile phase composed of Acetonitrile: Water (70:30 % v/v) [HPLC Grade] at flow rate 1ml/min with UV detection at 228 nm. The retention times of Metformin HCl and Myo-Inositol were found to be 2.752 min and 3.626 min respectively. Linearity was established for Metformin HCl and Myo-Inositol in the range of 25-150µg/ml and 60-360µg/ml respectively. System precision and method precision was found to be within the limits of the acceptance criteria. Relative standard deviation of Metformin HCl and MyoInositol for System precision was found to be 0.22 and 0.02 respectively and method precision was found to be 0.23 and 0.257 respectively. The percentage recoveries for Metformin HCl and Myo-Inositol were found to be in the range of 100.15-102.0% and 99.75-100.04% respectively. The limit of detection was 0.17µg/ml and 1.08µg/ml respectively. The limit of quantification was 0.52µg/ml and 0.37µg/ml respectively and the method was found to be specific. This method can be successfully employed for simultaneous quantitative analysis of Metformin HCl and Myo-Inositol in bulk drugs and formulations. The results indicate that there is no interference from excipients for the proposed method, thus making the method simpler, less time consuming and suitable for routine estimation of Metformin HCl and MyoInositol tablet formulation.

[Full Text Article]