WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

T. Y. Loo*, Xu Chunjiao and Mary N. B. Cheung

ABSTRACT

Objective: C-reactive protein is a sensitive and dynamic systemic marker of inflammation. Pro- and antiinflammatory cytokines have been well documented with initiation, control and susceptibility to periodontal diseases. This study applied high-sensitivity CRP (Hs-CRP), pro- and anti-inflammatory cytokines to predict risk of periodontal diseases. Methods: 2400 subjects (1200 healthy subjects and 1000 chronic periodontal patients) were recruited from Hong Kong, Xiangya School of Satomatology, Central South University, Changsha, China, respectively. Blood was drawn from subjects and DNA was extracted. The polymorphic sites of CRP, pro-inflammatory (interleukin-1? (IL-1?), IL-1?, tumor necrosis factor-? (TNF-?), IL-6 and IFN-?) and anti-inflammatory cytokines (IL-4, IL-10) were amplified and measured via polymerase chain reaction for further analysis. Chi-square test and logistic regression analysis were applied to analyze genotype distribution differences, allele frequencies and carriages rates between healthy and disease groups. Soluble protein levels of cytokines were evaluated by the t-test. Results: Genotype frequencies of CRP, pro- and anti-inflammatory cytokines was statistically higher in periodontal-diseased patients than healthy controls (p

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