WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals
World Journal of Advance Healthcare Research (WJAHR) has indexed with various reputed international bodies like : Google Scholar , Index Copernicus , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , Cosmos Impact Factor , Scientific Indexing Services (SIS) , UDLedge Science Citation Index , International Impact Factor Services , International Society for Research Activity (ISRA) Journal Impact Factor (JIF) , IFSIJ Measure of Journal Quality , Scientific Journal Impact Factor (SJIF) , International Scientific Indexing, UAE (ISI) (Under Process) , International Impact Factor Services (IIFS) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

ISSN 2457-0400

Impact Factor  :  5.464

News & Updation

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in WJAHR coming Issue.

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • 6th International Conference on Human and Soci

    Venue:FCT Education Resource Center, Abuja- Nigeria                                        September 22-24, 2019

  • WJAHR New Impact Factor

    Its our Pleasure to Inform you that WJAHR Impact Factor has been increased from  4.897 to 5.464 due to high quality Publication at International Level

  • .

    6th INTERNATIONAL CONFERENCE ON EDUCATIONAL TECHNOLOGY AND CURRICULUM STUDIES(ICETC2019) 

     

    Venue: FCT Education Resource Center, Abuja-Nigeria

    September 22-24, 2019

  • New Issue Published

    Its Our pleasure to inform you that, WJAHR 1 March 2021 Issue has been Published, Kindly check it on http://www.wjahr.com/home/current_issues

  • WJAHR MARCH ISSUE PUBLISHED

    MARCH 2021 Issue has been successfully launched on 1 March 2021.

Best Paper Awards

World Journal of Advance Healthcare Research (WJAHR)Honored the authors with best paper award, monthly based on the innovation of research work. Best paper will be selected by our expert panel.

Best Article of current issue

Download Article : Click here

Indexing

Abstract

A COMPARATIVE STUDY OF RAPID IMMUNO CHROMATOGRAPHY TEST WITH ELISA FOR DETECTION OF DENGUE NS1 ANTIGEN, IGM AND IGG ANTIBODY

Dr. R. Nagalakshmi*, Dr. V. G. Sridevi and Dr. C. Meenakshi

ABSTRACT

Aim of The Study: To analyse the efficacy of Immunochromatography with ELISA for detection of Dengue parameters (NS1 antigen, IgM and IgG antibodies) in Dengue suspected cases. Materials and Methods: A total of 100 blood samples obtained from adults with fever >4 days presenting with thrombocytopaenia (<1,00,000) were taken for the study from the Department of Medicine, Government Medical College Hospital, Tirunelveli after obtaining informed written consent. The samples were tested by Rapid Immunochromatography (ICT) and ELISA methods for NS1 Ag, IgM and IgG Ab and the results were evaluated for efficacy. RESULTS: Out of 100 adults tested by rapid ICT, 38% were positive for Dengue infection (NS1 antigen, IgM and IgG antibodies) in the serum sample. The same samples were tested by ELISA (Positive for any one of the three parameters) the positivity was found to be 59%. 37 samples positive by ICT were positive by ELISA also. Rapid ICT had a sensitivity of 87.5%, Specificity of 98.8% for NS1 antigen when evaluated against NS1 antigen. ELISA as gold standard. Rapid ICT had a very low sensitivity of 28.6% and 47.1% and an excellent specificity of 98% and 100% for IgM and IgG antibody detection when evaluated against MAC ELISA and GAC ELISA as gold standard respectively.13.6% showed primary infection and 64.4% showed secondary infection. Conclusion: Out of the total 100 cases studied, the rapid immunochromatography test has very less sensitivity and specificity is satisfactory. All samples should be subjected to both antigen (NS1) and antibody (IgM and IgG) testing to increase the positivity rate and to prevent the positive cases being missed. Cases with higher degrees of suspicion are to be subjected to diagnostic tests with higher sensitivity & specificity like ELISA and PCR. The commercially available rapid immunochromatographic test device can be used as a screening device during Dengue outbreaks. It should not be used as a standalone device for diagnosis of Dengue. Further molecular studies are essential to know the accurate information of Dengue serotypes which will be helpful in formulating vaccines in future.

[Full Text Article]