WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

An International Peer Review Journal for Medical Science and Pharma Professionals
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Indexing

Abstract

PHYTOCHEMICAL INVESTIGATIONS AND ANTI-CANCER ACTIVITY OF METHANOLIC EXTRACT OF ADINA CORDIFOLIA

Jayshri H. Bairagi* and Dr. R. S. Ghosh

ABSTRACT

Adina cordifolia is a deciduous tree of subfamily Cinchonoideae, family Rubiaceae, is found Southern Asia, from India and Srilanka east to southern China and Vietnam. It had been also shows anti-ulcer potential, active constituent showed interesting H+/K+ATPase inhibitory activity. The isolated stem of Adina cordifolia were identified as stigmasta-5,22-diene-3P-O-a-rhamnopyranosyl-(1-4)-P-Dxylopyranoside, a-amyrin, octacosanol and naringenin-7-methylether-4’-O-a-rhamnopyranoside on the basis analytical elucidation. Tannins and Phenolic compounds, Flavonoids are significantly present in 70% Hydroalcoholic and methanolic extract whereas the alkaloids, Triterpenoids, Sterols, Amino acids and Proteins presence were found negligible by the colour reactions. TLC profile of the methonolic extract for flavonoids and coumarins showed seven spots after acid spray and heated up to 1100C, whereas six spots were seen, when observed under UV light at 365 nm, before acid spray using n- Butanol: Acetic Acid: water (4:1:5) as mobile phase. Whereas TLC profile of the methonolic extract for coumarins showed three spots after acid spray and heated up to 1100C,whereas single spot was seen, when observed under UV light at 365 nm, before acid spray. using 10% Acetic Acid as mobile phase. Anti-proliferative activity of methanolic extract of Adina cordifolia, was determined using cell lines. Cells (5 × 103) were used various concentrations of Adina cordifolia, extracts (0-100 ?g/ml) were added. The IC50 values were calculated by plotting the percentage survival versus the concentration of extract.

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