WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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  • Index Copernicus
  • SOCOLAR, China
  • Research Bible, Fuchu, Tokyo. JAPAN
  • Cosmos Impact Factor
  • Scientific Indexing Services (SIS)
  • UDLedge Science Citation Index
  • International Impact Factor Services
  • International Society for Research Activity (ISRA) Journal Impact Factor (JIF)
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Abstract

PHYTOCHEMICAL SCREENING, DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR ISOLATED STIGMASTEROL FROM OCIMUM SANCTUM

Apporva Chawla*, Atinderpal Singh, Shaina Sharma, Varinder Soni

ABSTRACT

Stigmasterol is the abundant phytoconstituent present in the leaves of Ocimum sanctum, having a major function to maintain the structure and physiology of cell membranes. The dried leaves of Ocimum sanctum was subjected to extraction, preliminary phytochemical screening and then isolation. The identification of the isolated compound was confirmed by TLC, HPLC profile and spectral studies by comparing with the standard. RP-HPLC method were developed and validated as per ICH guidelines using the isolated biomarker compounds such as stigmasterol. Results of HPLC analysis of isolated compound shows the presence of stigmasterol which is confirmed by the single peaks present in the chromatogram at 3.18 retention time (in mins). The reported data may be of great value as a reference standard for evaluation of the isolated compound.

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