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Abstract
ISOLATION, IDENTIFICATION, AND ANTIMICROBIAL SUSCEPTIBILITY TESTING OF KLEBSIELLA PNEUMONIAE FROM PNEUMONIA PATIENTS
Bashir H. Hassoun*, Ghaidaa J. Mohammed
ABSTRACT
Gram-negative bacteria, Klebsiella pneumoniae is an important opportunistic pathogen that causes a variety of infectious diseases in humans, including pneumonia, liver abscesses, diarrhea, and septicemia. The study's aimed to isolate and identify Klebsiella pneumoniae from pneumonia patients in Al-Diwanyiah teaching hospital and Al-Diwanyiah maternity and children teaching hospital, testing the sensitivity of isolates to antibiotics as well as to molecular detection Klebsiella pneumoniae isolates by PCR. The study extended from December 2024 and April 2025. Biochemical tests, Vitek 2 and 16S rRNA identified 36 bacteria as Klebsiella pneumoniae from 200 samples. Antibiotic susceptibility of Klebsiella pneumoniae isolates against 8 of commonly used antibiotic was determined through disc-diffusion method. Results declared that the isolates were resistant to the used antibiotics except their sensitivity to Imipenem, Meropenem and Tigecycline.
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