WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

Sara Fawzi Sahm*

ABSTRACT

One of the three main dietary groups required for a healthy diet is protein. The class of enzymes called proteolytic enzymes, also called proteinases, is responsible for proteins' catalytic hydrolysis. Bacillus licheniformis Strain Sara produces protease enzyme and partially purifies it was the study's objective. Protease is the most valuable microbial enzymes after amylase. Protease have been used extensively and in a variety of applications due to their ability to break down complex proteins into simple proteins or short peptides. Current research focuses on Purification, characterization, and application of proteases isolated from Bacillus licheniformis Strain Sara species. Fractional Ammonium sulfate precipitation was administered in different quantities to induce the precipitation of enzymes. In purification system, anionic exchange simple technique was utilized by using column to separate protease from other proteins. The necessary partially purified protease molecular weight was determined using SDS-PAGE with 8-fold purity and 6.9 % yield. Molecular weight of the protease estimated to be around 36 kDa. Along with this partially purified protease used in variety of application like production of small peptides from soy protein and antimicrobial activity of that small soy protein petides.

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