WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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  • Google Scholar
  • Index Copernicus
  • SOCOLAR, China
  • Research Bible, Fuchu, Tokyo. JAPAN
  • Cosmos Impact Factor
  • Scientific Indexing Services (SIS)
  • UDLedge Science Citation Index
  • International Impact Factor Services
  • International Society for Research Activity (ISRA) Journal Impact Factor (JIF)
  • IFSIJ Measure of Journal Quality
  • Scientific Journal Impact Factor (SJIF)
  • International Scientific Indexing, UAE (ISI) (Under Process)
  • International Impact Factor Services (IIFS)
  • Web of Science Group (Under Process)
  • Directory of Research Journals Indexing
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Abstract

INVESTIGATION OF THE PREVALENCE OF CARBAPENEM RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES ISOLATED FROM DIFFERENT CLINICAL SAMPLES IN AL-DIWANIYAH GOVERNORATE

*Farah Awid Abed, Fatima Raheef and Dhuha M. Jabir* and Salam Jasim Mohammed

ABSTRACT

Back ground: Carbapenem is the last choice treatment for multi-antibiotic resistant Pseudomonas aeruginosa infections and the spread of resistant strains to this antibiotic is a serious problem. Methods: In the current study, 250 clinical samples were collected from different pathological cases in Al-Diwaniyah governorate, distributed as follows: 50 smears for burn injuries, 50 samples from the intensive care unit, 50 samples for wounds, 50 sputum samples for patients with acute pneumonia, and 50 urine samples for patients suffering from urinary tract infection. all collected samples were cultured on the blood and MacConkey agar medium. the bacteria were diagnosed by biochemical methods and staining. Pseudomonas aeruginosa were isolated, then an antimicrobial sensitivity test was performed using Vitek -2 compact system (BioMérieux Company \France). At last the ability of carbapenem-resistant bacteria to produce the carbapenemase enzyme was investigated by the NitroSpeed-Carba NP test. Results: the results showed that there were 70 carbapenem-resistant isolates(28%), and most of the isolates were from infection and wound patients(n= 40, 16%), while the fewest isolates were from burn injuries(n=10). The study also found that the most produced carbapenemase was the class D. Conclusion: There is a significant proportion of isolates that are resistant to carbapenems, because they produce carbapenase enzyme class D.

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