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Abstract
IDENTIFICATION OF KEY GENES IN INTERVERTEBRAL DISK DEGENERATION USING AN IN VIVO RAT MODEL
Yi Wang*, Yan Xu, Ling Jiang, Yanjie Wang, Guogang Dai and Hai Shen
ABSTRACT
In the present study, we established a rat model of intervertebral disc degeneration (IDD). RNA sequencing was used to identify differentially expressed genes (DEGs) between the intervertebral discs of IDD model and control rats. Metascape website was used for bioinformatics analyses, and the key genes in IDD were identified. The expression of key genes was examined by qRT?PCR and immunofluorescence staining. A total of 586 DEGs were identified between the IDD and control groups. Bioinformatics analysis revealed that these DEGs were enriched in 13 GO MF terms, 28 GO CC terms, 136 GO BP terms, 33 KEGG pathways, and TNF-α, TLR2, ITGAM, TTN and TCAP were identified as key genes in IDD. QRT?PCR and immunofluorescence staining revealed that the expression levels of TNF, TLR2, ITGAM and TTN were greater in degenerated disc tissues than in normal disc tissues, whereas the expression level of TCAP was lower in degenerated disc tissues than in normal disc tissues. In conclusion, our study revealed that TNF-α and TLR2 are key genes in IDD, and that ITGAM, TTN, TCAP and the calcium signaling pathway may play key roles in IDD. Our research may provide new clues for future studies exploring the pathogenesis of IDD.
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