WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

Choupette Ravelle Dobhat-Doukakini*, Landry Martial Miguel, Childérick Lekana, Destin Maurélien Mbemba Bahamboula, Donatien Moukassa and Ange Antoine Abena

ABSTRACT

Neuroinflammation is described as a key mechanism in the onset and/or progression of several neurological disorders and is the subject of several researches. Several protocols have been proposed to model this condition, such as LPS injection, which induces neurodegeneration and anxiety-like behavior. Numerous studies have been carried out to understand the pathophysiological mechanisms of neuroinflammation. The aim of this study was to evaluate the effects of DHA administration on behavioral disorders as well as tissue damage induced by LPS in mice. Thirty male Balb /c strain mice weighing between 11.7 and 30.2g were randomly divided into three groups (n=10). The first group received (distilled water, 1ml/Kg). Neuroinflammation was induced in groups 2 and 3 by administration of 500 µg/Kg, ip of LPS for one week. Twenty-four hours after induction of neuroinflammation, the animals received water and DHA (55 mg/kg, per os), respectively. Finally, twenty-four hours after DHA treatment, the animals were sacrificed and the brains collected for histological analysis. The results obtained show that LPS significantly reduces motor activity. However, the administration of DHA at a dose of 55 mg/kg does not significantly increase this activity. Concerning the anxiety index, the results show a non-significant increase in the latter after treatment with DHA. Histologically, our behavioral results are in agreement with histological examination, which revealed that tissues from DHA-treated mice exhibited neurons and macrophage cells containing a pale vesicular nucleus and an obvious nucleolus (in the form of a perinuclear halot), as well as dark, polymorphonuclear cells, degenerating with phagocytic effect, unlike the tissues of the other two groups. In conclusion, our results suggest that administration of 55 mg/kg of DHA has no significant effects on behavior, but histological examination shows progressive repair.

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