WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals
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ISSN 2457-0400

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Indexing

Abstract

PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTHELMINTIC ACTIVITY OF LAWSONIA INERMIS LEAVES

Dr. Praveen Kumar Dasari*, Bondili Sadhana, Tatina Anju and Guduru Sai Krishna

ABSTRACT

The present research work deals with phytochemical investigation and in-vitro anthelmintic activity of lawsonia inermis leaves on Indian earthworms, Pheretima posthuma having physiological and anatomical similarity with intestinal helminthes. Standardization of crude drugs isolated from plant plays a vital role in identification of the quality and purity of drugs. Now a day parasitic (helminthes) infections are growing rapidly worldwide and numerous synthetic antihelmintic drugs are in use for the treatment of helminthiasis but they posses some allergic manifestations as well as adverse effects, in order to overcome that effects natural anthelmintic agents are required in present scenario. This research observed and highlights that ethanolic extracts of lawsonia inermis leaves had the highest number of phytochemicals compared to other solvent extracts. Hence, ethanolic extracts of lawsonia inermis leaves holds the greatest potential to treat various human diseases and has profound medical applicability. The adult worms are collected and cleaned in normal saline solution before they were placed in to respective formulation. To observe anthelmintic activity, all the investigations were carried out by ethanol, acetone and aqueous extract with different concentrations of 10, 25, 50mg/ml. A significant activity was observed at highest concentrations of 50mg/ml of ethanolic extract and compared with albendazole (10, 25, 50mg/ml) as standard.

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