WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

World Journal of Advance Healthcare Research (WJAHR) has indexed with various reputed international bodies like : Google Scholar , Index Copernicus , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , Cosmos Impact Factor , Scientific Indexing Services (SIS) , UDLedge Science Citation Index , International Impact Factor Services , International Society for Research Activity (ISRA) Journal Impact Factor (JIF) , IFSIJ Measure of Journal Quality , Scientific Journal Impact Factor (SJIF) , International Scientific Indexing, UAE (ISI) (Under Process) , International Impact Factor Services (IIFS) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Academia , Scope Database , 

ISSN 2457-0400

Impact Factor  :  6.711

News & Updation

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in WJAHR coming Issue.

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • 6th International Conference on Human and Soci

    Venue:FCT Education Resource Center, Abuja- Nigeria                                        September 22-24, 2019

  • .

    6th INTERNATIONAL CONFERENCE ON EDUCATIONAL TECHNOLOGY AND CURRICULUM STUDIES(ICETC2019) 

     

    Venue: FCT Education Resource Center, Abuja-Nigeria

    September 22-24, 2019

  • WJAHR: New Impact Factor

    WJAHR Impact Factor has been Increased to 6.711 for Year 2024.

  • New Issue Published

    Its Our pleasure to inform you that, WJAHR March 2023 Issue has been Published, Kindly check it on https://www.wjahr.com/home/current_issues

  • WJAHR: MARCH ISSUE PUBLISHED

    March 2024 Issue has been successfully launched on 1 March 2024.

Best Paper Awards

World Journal of Advance Healthcare Research (WJAHR)Honored the authors with best paper award, monthly based on the innovation of research work. Best paper will be selected by our expert panel.

Best Article of current issue

Download Article : Click here

Indexing

Abstract

EVALUATION OF DNA OXIDATIVE DAMAGE BY 8-OHDG BIOMARKER DURING SPERM ACTIVATION WITH PLATELET-RICH PLASMA AND GLASS WOOL FILTRATION TECHNIQUES

Watfaa A. Abduljabar, Hayder A. L. Mossa* and Muayad S. Abood

ABSTRACT

Background: Seminal oxidative stress (OS) is known as one of the important factors of male infertility through pathogenesis of sperm dysfunction and DNA damage. Oxidative DNA damage can lead to multiple forms of mutations as a result of structural alterations of bases as well as helix distortions and the production of single and double-strand DNA breaks. The most common product of oxidative DNA damage is oxidation of guanine to form 8-hydroxyguanine (8-oxo-G) or 8-hydroxy-2' -deoxyguanosine (8-OHdG). If left unrepaired this base will pair with adenine instead of cytosine. So accumulation of 8-OHdG in DNA may result in various pathologies and the significantly altered level of base damage analyzed by 8-OHdG is a potentially early diagnostic biomarker for such pathologies. Objective: Detection of oxidative DNA damage of the spermatozoa by measuring the 8-OHdG biomarker during sperm activation with Platelet-Rich Plasma(PRP) and Glass Wool filtration(GWF) techniques. Patients and Methods: The study included 60 infertile men as (45Asthenozoospermic,15 Normozoospermic) which were involved, their semen samples were analyzed and then activated with PRP and filtered with GWF, during their attendance to the infertility clinic of the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, the 8-OHdG biomarker was measured for each semen sample, three(3) readings were obtained as: before activation, after Glass Wool Filtration and after Platelet -Rich Plasma with Glass Wool Filtration sperm activation techniques. Conclusion: After comparison between the two groups of infertile men and measuring 8-OHdG biomarker, the latter has been found to be less concentration in normozoospermic than asthenozoospermia which is sensitive indicator for DNA damage, the concentration of 8-OHdG was also less following activation with Platelet -Rich Plasma and Glass Wool Filtration, than its concentration before activation.

[Full Text Article] [Download Certificate]