WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals
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Indexing

Abstract

EVALUATION OF DNA OXIDATIVE DAMAGE BY 8-OHDG BIOMARKER DURING SPERM ACTIVATION WITH PLATELET-RICH PLASMA AND GLASS WOOL FILTRATION TECHNIQUES

Watfaa A. Abduljabar, Hayder A. L. Mossa* and Muayad S. Abood

ABSTRACT

Background: Seminal oxidative stress (OS) is known as one of the important factors of male infertility through pathogenesis of sperm dysfunction and DNA damage. Oxidative DNA damage can lead to multiple forms of mutations as a result of structural alterations of bases as well as helix distortions and the production of single and double-strand DNA breaks. The most common product of oxidative DNA damage is oxidation of guanine to form 8-hydroxyguanine (8-oxo-G) or 8-hydroxy-2' -deoxyguanosine (8-OHdG). If left unrepaired this base will pair with adenine instead of cytosine. So accumulation of 8-OHdG in DNA may result in various pathologies and the significantly altered level of base damage analyzed by 8-OHdG is a potentially early diagnostic biomarker for such pathologies. Objective: Detection of oxidative DNA damage of the spermatozoa by measuring the 8-OHdG biomarker during sperm activation with Platelet-Rich Plasma(PRP) and Glass Wool filtration(GWF) techniques. Patients and Methods: The study included 60 infertile men as (45Asthenozoospermic,15 Normozoospermic) which were involved, their semen samples were analyzed and then activated with PRP and filtered with GWF, during their attendance to the infertility clinic of the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, the 8-OHdG biomarker was measured for each semen sample, three(3) readings were obtained as: before activation, after Glass Wool Filtration and after Platelet -Rich Plasma with Glass Wool Filtration sperm activation techniques. Conclusion: After comparison between the two groups of infertile men and measuring 8-OHdG biomarker, the latter has been found to be less concentration in normozoospermic than asthenozoospermia which is sensitive indicator for DNA damage, the concentration of 8-OHdG was also less following activation with Platelet -Rich Plasma and Glass Wool Filtration, than its concentration before activation.

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