WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

Prabhusaran N.*, Prabhakar Y. K., Kalimuthu R. and Pramila M.

ABSTRACT

Environmental sampling including the swabs from the surface of various locations in food processing units are considered as the major area thereby the overall hygiene is get disturbed leads to complete contamination in the surface hygiene. The main objective of this study is to analyze the presence of residential bacterial population from the surface areas of different locations of food processing units including restaurants and fresh juice preparation shops. The swabs were taken from five different locations of the food processing units before and after cleaning and disinfection mainly of two time with two days differences. Microbial enrichment media was used for the immediate culturing and transport to the laboratory. General, selective and differential culture media were used to determine the bacterial population. As a result, before cleaning and disinfection, gram negative bacteria including Pseudomonas aeruginosa, Escherichia coli dominated followed by Micrococcus sp and Acinetobacter sp whereas Staphylococcus aureus was found largely in the places where more milk and its products used. The second samples after two days after cleaning and disinfection, Pseudomonas and Acinetobacter remains due to its biofilm forming nature and tolerance to biocides. Predominantly both the samples showed the same set of bacterial species to be grown due to its residential nature on the particular nutrition rich environment. Thus cleaning and disinfecting before starting the morning jobs in these environment is suggested. Same way, the type and concentration of the disinfectant and method of disinfection should be changed regularly in order to reduce or avoid the colonization of the residential bacteria that may or may not cause mild to severe infections to the consumers.

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