WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

Dr. Shubhangi Warke*

ABSTRACT

Next-generation sequencing (NGS) also referred to as deep, high- throughput or massively parallel sequencing, is a powerful new tool that can be used for complex diagnosis and intensive monitoring of infectious disease in veterinary medicine. NGS technologies are also being increasingly used to study the aetiology, genomics, evolution and epidemiology of infectious disease, as well as host -pathogen interactions, host immune responses, Complexity of infection biology, including responses to antimicrobial treatments and vaccination. NGS approaches can function as important tools for studying disease outbreaks by identifying and following transmission routes, thereby facilitating the identification of outbreak origins in zoonotic diseases. The topic covers the era of sequencing, steps in next gene sequencing, mainly various application of NGS in disease and cancer diagnosis, novel pathogen discovery, genome characterization and viral diversity, viral metagenomics, host pathogen interaction, chromatin immunoprecipitation and transcriptomics. Since the introduction of NGS platforms, continuous developments and improvements have resulted in update instruments with increases capacity to generate sequence data. Consequently, there has been a corresponding dramatic decrease in the cost per base. In Veterinary complex diseases such as post weaning multisystemic wasting syndrome, investigations of infections and diseases in cattle and sheep included characterizing and determining the phylogeny of new bluetongue virus variants ; determining host viral population diversity ; and detecting unknown and emerging new pathogens most notably the Schmallenberg virus characterisation and phylogenetic analysis of new variants of various viruses; the detection of unknown and unexpected pathogens; investigation of poultry microbiota; host pathogen interactions; in detecting and identifying some of the newly emerging variants of infectious agents have been studied using NGS.

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